GSM3581277: Δagr + DMSO rep1; Staphylococcus aureus subsp. aureus USA... - SRA

SRX5299660: GSM3581277: Δagr + DMSO rep1; Staphylococcus aureus subsp. aureus USA300; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 23.8M spots, 6G bases, 2Gb downloads

Submitted by: NCBI (GEO)

Study: Effect of apicidin on gene expression in Staphylococcus aureus USA300 strain LAC

PRJNA517434 • SRP182661 • All experiments • All runs

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The fungal metabolite apicidin acts as a quorum sensing inhibitor in Staphylococcus aureus. Here we use RNA-sequencing to examine the effect of apicidin on gene expression, comparing to untreated wild-type and an agr quorum sensing mutant. Overall design: Illumina RNA-sequencing of S. aureus USA300 LAC and LAC ?agr +/- 100 µM apicidin, in triplicate.

Sample: Δagr + DMSO rep1

SAMN10830408 • SRS4298616 • All experiments • All runs

Organism: Staphylococcus aureus subsp. aureus USA300

Library:

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: 1 h lysis step with lysostaphin followed by RNA purification using Qiagen RNeasy Mini Kit. rRNA depleted using Ribo-Zero rRNA Removal kit for Gram positive bacteria (Illumina) and libraries prepared using TruSeq Stranded mRNA Library Prep Kit (Illumina).

Experiment attributes:

GEO Accession: GSM3581277

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 23.8M spots, 6G bases, 2Gb

Run	# of Spots	# of Bases	Size	Published
SRR8495145	23,792,883	6G	2Gb	2019-02-04

ID:: 7173603

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